The log10 of the survivors was plotted against time to yield an inactivation curve for the three test sites, and regression analysis was used to determine the decimal reduction time ( D-value ) for each curve . 8 The D-value curves revealed a classical pattern of microbial inactivation : the interior of the single product package is more resistant than the exterior and the least accessible location within the product ( BI in the aspiration lumen ) is the most resistant location of the three tested.
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