Genome-wide knockdown studies are an example of the reverse genetics made possible by the acquisition of whole genome sequences, and the advent of genomics and gene-silencing technologies, mainly siRNA and deletion mapping.
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The principle of deletion mapping involves crossing a strain which has a point mutation in a gene, with multiple strains who each carry a recombination occurs between the two strains to produce a wild-type ( + ) gene ( regardless of frequency ), the point mutation cannot lie within the region of the deletion.
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