1. The priming event on the lagging strand establishes a replication fork. 2. The lagging strand is synthesized in short, separated segments. 3. In contrast, lagging strand synthesis is accomplished in short Okazaki fragments. 4. The leading strand receives one RNA primer while the lagging strand receives several. 5. A DNA polymerase I molecule ( green ) binds to the lagging strand . 6. DNA ligase then joins the deoxyribonucleotides together, completing the synthesis of the lagging strand . 7. However, there is a problem going in the other direction on the lagging strand . 8. However, significant challenges remain : the leading and lagging strands are anti-parallel. 9. On the leading strand this route is continuous, but on the lagging strand it is discontinuous. 10. During lagging strand synthesis, the replicative polymerase sends the lagging strand back toward the replication fork.
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