In scattering tissue, on the other hand, the superior optical sectioning and light detection capabilities of the two-photon microscope result in better performance.
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This method is based on the determination of pixel-level Forster resonance energy transfer ( FRET ) efficiency in conjunction with spectrally resolved two-photon microscope.
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Image Correlation Spectroscopy and its variations all were implemented on a scanning confocal or scanning two photon microscope, but transfer to other microscopes, like a spinning disk confocal microscope.
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The experiments reported by Hyman's group at MGH depended on the new microscope, called a multi-photon microscope, which Hyman said " takes up half a room ."
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