Newcombe replica plated it onto two new Petri dishes treated with phage.
"' Replica plating "'is a streaking each out individually onto a separate plate.
Replica plating is especially useful for " negative selection ".
He was also presented with a replica plate as a permanent reminder of the occasion.
In Falkow's case, this happened a few years after she first published the replica plating paper.
At the memorial for Esther Lederberg, he spoke of the impact of replica plating, and his feelings upon meeting the originator of the technique:
Two and a half miles ( 4 kilometers ) above, on the white linen tables of the Keldysh, other tourists dine off replica plates.
The purpose of replica plating is to be able to compare the master plate and any secondary plates, typically to screen for a desired phenotype.
For example, if one wanted to select colonies that were sensitive to ampicillin, the primary plate could be replica plated on a secondary Amp + agar plate.
The method of replica plating implemented by Esther Lederberg included auxotrophs that were temperature-sensitive; that is, their ability to synthesize was temperature-dependent . ( Auxotrophs are usually not temperature-dependent.
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