Rho factor is an essential transcription protein in prokaryotes.
2.
A Rho factor acts on an RNA substrate.
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In short, Rho factor acts as an ATP-dependent unwinding enzyme, moving along the newly forming RNA molecule towards its 32 end and unwinding it from the DNA template as it proceeds.
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So, even though the RNA polymerase is about 40 nt per second faster than Rho, it does not pose a problem for the Rho termination mechanism as the RNA polymerase allows Rho factor to catch up.
5.
They are consistently located in what could be the 5'untranslated regions of genes that encode the Rho factor protein, and this arrangement in bacteria suggested that " Pseudomon "-Rho RNAs might be cis-regulatory elements that regulate concentrations of the Rho protein.
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