Potassium ferricyanide is used in many amperometric biosensors as an electron transfer agent replacing an enzyme's natural electron transfer agent such as oxygen as with the enzyme glucose oxidase.
12.
In general, there are several approaches that can be used to detect electrochemical changes during a biorecognition event and these can be classified as follows : amperometric, potentiometric, impedance, and conductometric.
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In pulsed amperometric detection ( PAD ), a working potential is applied for a short time ( usually a few hundred milliseconds ), followed by higher or lower potentials that are used for cleaning the electrode.
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An exception to the usage of placing the optic fibre transdermally is seen in the previously mentioned fibre from Ingo Klimant's group ( ruthenium-quenching by oxygen released by glucose-catalysed GOx ) : The sensor, in fact, was constructed by functionalizing a premade oxygen sensor with Glucose oxydase and inserting it into a glucose-sensing apparatus for amperometric sensors, in particular, a microdialysis catheter CMA 60 implanted transdermally and the sensor connected to its tygon tubing.
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