In the case of the baker's or brewer's yeast " Saccharomyces cerevisiae " growing on glucose with plenty of aeration, the diauxic growth pattern is commonly observed in batch culture.
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For example, in a mixed batch culture the target species may die or have reduced growth rates due to competition for nutrients, dissolved inorganic carbon, or pH levels which are too low for the target species.
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In a batch culture, to achieve very high cell concentrations, " e . g . " 50-100 g of dry cells / L, high initial concentrations of the nutrients in the medium are needed.
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A powerful method of overcoming the catabolite repression in the enzyme biosynthesis is a fed-batch culture in which glucose concentration in the culture liquid is kept low, where growth is restricted, and the enzyme biosynthesis is derepressed.
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The inset shows that the change in the number of CFUs between day 4 and day 1 depends on the initial GASP fraction of a culture . ( b ) Growth curves, measured as optical density ( OD ) at 600 nm, of well-mixed batch cultures.
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