The rate of branch migration varies dramatically with ion concentration, with single-step times increasing from 0.3 " 0.4 ms with no ions to 270 " 300 ms with 10 mM Mg 2 +.
12.
*" These enzymes are attracted to double strand breaks, search for sequence similarity between the duplex strands and catalyze formation of a Holliday junction, branch migration, and resolution . " This sentence is a big jumble.
13.
This complex nucleates with hairpin H2 by means of base pairing to toehold 3, mediating a branch migration that opens the hairpin to form complex I�H1�H2 containing single-stranded segment 2 *-1 * .
14.
The pathways are also similar in their phases of " branch migration ", in which the Holliday junction slides in one direction, and " resolution ", in which the Holliday junctions are cleaved apart by enzymes.
15.
Initiator I nucleates with hairpin H1 via base pairing to single-stranded toehold 1, mediating a 3-way branch migration that opens the hairpin to form complex I�H1 containing single-stranded segment 3 *-2 * .
16.
B assembles with C ( step 2 ) via loop / toehold nucleation and 3-way branch migration to form Dicer substrate B�C . Chemical modifications ( 2'OMe-RNA ) prevent degradation : A and part of C ( dashed backbone ).
17.
In more recent years, however, this view has been challenged, and most of the current models for Int, Xer, and Flp recombination involve only limited branch migration 1 3 base pairs ) of the Holliday intermediate, coupled to an isomerisation event that is responsible for switching the strand cleavage specificity.
18.
Additionally, the frequency of gene conversion is inversely proportional to the distance between the interacting sequences in cis ( Schildkraut et al ., 2005 ), In the studies of human globulin genes, it has long been supported that the gene conversion event or branch migration events can either be promoted or inhibited by the specific motifs that exist in the vicinity of the DNA sequence ( Papadakis and Patrinos, 1999 ).
19.
Conditional Dicer substrate formation via shape and sequence transduction with small conditional RNAs . scRNA A�B detects mRNA detection target X ( containing subsequence a-b-c ), leading to production of Dicer substrate B�C targeting mRNA silencing target Y ( containing independent subsequence w-x-y-z ) . scRNAs A�B and C are stable in the absence of X . A swaps B for X ( step 1 ) via toehold-mediated 3-way branch migration and spontaneous dissociation.
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