11. For the flushing circulation a buffer solution is used. 12. IUPAC has proposed the use of a set of buffer solutions of known H + activity. 13. The apparatus includes a U-shaped cell filled with buffer solution and electrodes immersed at its ends. 14. The main conductance is 1nS in buffer solution . 15. The purified protein is usually dissolved in a buffer solution and adjusted to the desired solvent conditions. 16. In biochemistry equilibrium constants are often measured at a pH fixed by means of a buffer solution . 17. A buffer solution of sodium acetate and acetic acid I believe, has a pH of around 4.76. 18. If a pH of exactly 7.0 is required, it must be maintained with an appropriate buffer solution . 19. The pH meter is usually calibrated with buffer solutions at known pH values before starting the titration. 20. Agarose gels are cast horizontally in a mold, and when set, usually run horizontally submerged in a buffer solution .