Third, spots of each cDNA clone or oligonucleotide are present as replicates ( at least duplicates ) on the microarray slide, to provide a measure of technical precision in each hybridization.
12.
Performing a BLASTn query search with the ESTs ( expressed sequence tags ) database for the cDNA clones derived from the probes, revealed that 53 % of related transcripts were found in placental cells.
13.
Later ligand-binding studies provided direct evidence of binding sites for baclofen on central neuronal membranes . cDNA cloning confirmed that the GABA B receptor belongs to the family of G-protein coupled receptors.
14.
Since a cDNA clone of this gene contains a 119 bp-region in the 5'UTR, it is likely that " HSPA1L " contains one or more introns in its own 5'UTR.
15.
Finally, by overlapping cDNA cloned fragments, they were able to determine that the C4 loci, an estimated 16 kilobase ( kb ) long, are spaced by 10 kb and aligned 30 kb from the factor B locus.
16.
He pioneered the method of cDNA cloning, an approach universally used in bioscience and biotechnology, and elucidated the organization, diversity and rearrangement of human antibody genes, which defined the building blocks for construction of therapeutic antibody repertoires.
17.
Maniatis was appointed to the position of assistant professor in the Department of Biochemistry and Molecular Biology in 1975, but carried out his research at Cold Spring Harbor Laboratory because the moratorium on recombinant DNA research in Cambridge, MA prevented his work on cDNA cloning at Harvard.
18.
The hepatitis C virus was known to exist ( as " non A, non B viral hepatitis " ) for more than a decade before Michael Houghton's group at Chiron discovered it by screening over a million cDNA clones in a talk ) 18 : 39, 19 April 2009 ( UTC)
19.
DNA for cloning experiments may also be obtained from RNA using reverse transcriptase ( complementary DNA or cDNA cloning ), or in the form of synthetic DNA ( artificial gene synthesis ) . cDNA cloning is usually used to obtain clones representative of the mRNA population of the cells of interest, while synthetic DNA is used to obtain any precise sequence defined by the designer.
20.
DNA for cloning experiments may also be obtained from RNA using reverse transcriptase ( complementary DNA or cDNA cloning ), or in the form of synthetic DNA ( artificial gene synthesis ) . cDNA cloning is usually used to obtain clones representative of the mRNA population of the cells of interest, while synthetic DNA is used to obtain any precise sequence defined by the designer.
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