The probe protein is often produced in " E . coli " using an expression cloning vector.
12.
In cases where DNA fragments are cloned before sequencing, the resulting sequence may contain parts of the cloning vector.
13.
In contrast, PCR-based cloning and next-generation sequencing technologies based on pyrosequencing often avoid using cloning vectors.
14.
The cloning vector is treated with a restriction endonuclease to cleave the DNA at the site where foreign DNA will be inserted.
15.
Modern cloning vectors include selectable antibiotic resistance markers, which allow only cells in which the vector has been transfected, to grow.
16.
It has two antibiotic resistance genes, and a number of convenient unique restriction sites that made it suitable as a cloning vector.
17.
Other cloning vectors may use topoisomerase instead of ligase and cloning may be done more rapidly without the need for restriction digest of the vector or insert.
18.
Additionally, the cloning vectors may contain colour selection markers, which provide blue / white screening ( alpha-factor complementation ) on X-gal medium.
19.
Naturally occurring circular plasmids can be modified to contain multiple resistance genes and several unique restriction sites, making them valuable tools as cloning vectors in biotechnology applications.
20.
The primers may have additional sequences added to their 5'ends, such as sequences for restriction enzyme recognition sites needed for ligation into a cloning vector.
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