In a pioneering study by Bernstein and Bamburg, it was observed that the actin-binding protein " actin depolymerisation factor ( ADF ) / cofilin ", a factor that promotes actin filament depolymerisation, competed with tropomyosin for binding to the actin filament.
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Depolymerisation is an indirect method of analysis allowing to gain informations such as average degree of polymerisation, percentage of galloylation, etc . The depolymerised sample can be injected on a mass spectrometer with an electrospray ionization source, only able to form ions with smaller molecules.
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