The excited molecules will transfer the energy they now possess to the scintillator molecules, where the energy will be emitted as light.
12.
Birks speculated that the loss of linearity is due to recombination and quenching effects between the excited molecules and the surrounding substrate.
13.
Hence, if an excited molecule transfers its energy to a sensitizer thus exciting it, longer and easier to quantify light emission is often observed.
14.
When the excited molecule has a nearby neighbour molecule, the excitation energy may also be transferred, through electromagnetic interactions, from one molecule to another.
15.
Collisions with other molecules cause the excited molecule to lose vibrational energy until it reaches the lowest vibrational state of the excited electronic state.
16.
The ionization process involves an interaction between the analyte molecule ( S ) and electronically excited atoms or vibronically excited molecules ( metastable species M * ):
17.
When an electron in an excited molecule or atom descends to a lower energy level, it emits a photon of light at a frequency corresponding to the energy difference.
18.
When polarized light is applied to a group of randomly oriented fluorophores, most of the excited molecules will be those oriented within a particular range of angles to the applied polarization.
19.
In contrast the method of Polanyi measures vibrational energy of the products by detecting the infrared chemiluminescence emitted by vibrationally excited molecules, in some cases for reactants in defined energy states.
20.
On the other hand, if the emission polarizer is horizontally polarized there will be an additional introduction of excited molecules that were initially vertically polarized and became depolarized via Brownian motion.
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