Scientist Joan Knoll sat at a fluorescence microscope, studying what looked like tiny strands of spaghetti with bands wrapped around them.
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In this method, the antigens on the surface of the erythrocytes are marked with fluorescence and examined under a fluorescence microscope.
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As noted above, CISH is much cheaper and is easier to use because it uses bright-field microscopes instead of fluorescence microscopes.
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:: : If you happen to have access to a fluorescence microscope, there are several yeast-specific Live / Dead staining kits available.
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A fluorescence microscope can detect the dyes present and send it to a computer that can reveal the karyotype of a cell.
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The "'Simulated Fluorescence Process "'( SFP ) is a computing algorithm used for scientific visualization of 3D data from, for example, fluorescence microscopes.
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Apart from the expensive fluorescence microscope, FISH also requires a high-resolution digital camera to capture micrographs of the sample before the fluorescence fades.
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In preparation for imaging with a fluorescence microscope, different methods can be used, such as fluorophores or dyes, immunolabeling, and genetically encoded fluorescent proteins.
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The primary antibody allows visualization of the protein under a fluorescence microscope when it is bound by a secondary antibody that has a conjugated fluorophore.
20.
Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent inverted fluorescence microscope that allows imaging of fluorescence in cells.
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