11. The less obvious answer is horizontal gene transfer , which you mention. 12. Horizontal gene transfer makes it more difficult to study the last universal ancestor. 13. In the process of gene transfer , allelic gene conversion is biased. 14. Gene transfer strategies aim to target the disease process rather than the symptoms.15. Large-scale gene transfer has also occurred between the ancestors of mitochondria. 16. Most likely, these genes were acquired through horizontal gene transfer from viral hosts. 17. Hyperleptinemia produced by infusion or adenoviral gene transfer decreases blood pressure in rats. 18. A non-randomised safety and efficacy trial of a gene transfer vector is underway. 19. Repeat cycles of gene transfer and conversion increasingly make gene family members more similar. 20. VS ribozyme undergo horizontal gene transfer with other Neuropora strains.