11. DNA hybridization testing with Affirm VPIII was compared to the Gram stain using the Nugent criteria. 12. Bacteriology : microscopic image of a mixture of two types of bacteria stained with the Gram stain . 13. The Gram stain is almost always the first step in the preliminary identification of a bacterial organism. 14. These will appear positive by Gram stain even though they are not closely related to other gram-positive bacteria. 15. The Gram stain identifies the bacterial groups Firmicutes and Actinobacteria, both of which contain many significant human pathogens. 16. Gram stain / smear becomes definitive in the identification of rare, atypical or and possibly more serious organisms.17. Iodine is often referred to as a mordant in Gram stains , but is in fact a trapping agent. 18. Specimen adequacy is assessed by the laboratory technologists by examining a Gram stain or cytology stain of the sputum. 19. A Gram stain is first performed to guide the way, which should show typical gram-positive bacteria, cocci, in clusters. 20. :For bacteria the good old gram stain ( crystal violet / fuchsine ) is most likely what you're looking for.