"' Denaturation "'is a process in which proteins or nucleic acids lose the quaternary structure, tertiary structure and secondary structure which is present in their native state, by application of some external stress or compound such as a strong acid or aggregation due to the exposure of hydrophobic groups.
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Examples of compounds that reduces the solubility include many organic thiols and nitrogen compounds that do not possess solubilizing group other than mercapto group or the nitrogen site, such as mercaptooxazoles, mercaptotetrazoles, especially 1-phenyl-5-mercaptotetrazole, benzimidazoles, especially 2-mercaptobenzimidazole, benzotriazole, and these compounds further substituted by hydrophobic groups.
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