*An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up.
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The oil is applied to the specimen ( conventional microscope ), and the stage is raised, immersing the objective in oil . ( In inverted microscopes the oil is applied to the objective ).
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The inverted microscope that Hayflick modified for use in his tissue culture and mycoplasma work and on which all other such microscopes have been modeled has been acquired by the Smithsonian National Museum of American History.
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The first child born from a gamete micromanipulation ( technique in which special tools and inverted microscopes are used that help embryologists to choose and pick an individual sperm for ICSI IVF ) was a child in Singapore-born in April 1989.
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Oil immersion objectives can have practical difficulties due to their shallow depth of field and extremely short working distance, which calls for the use of very thin ( 0.17mm ) cover slips, or, in an inverted microscope, thin glass-bottomed Petri dishes.
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