11. It is the keto acid produced by deamination of glutamate, and is an intermediate in the Krebs cycle. 12. A very common ?-keto acid is ?-ketoglutarate, an intermediate in the citric acid cycle. 13. Many transamination reactions occur in tissues, catalysed by transaminases specific for a particular amino / keto acid pair. 14. Transamination is demonstrated if the corresponding new amino acid and keto acid are formed, as revealed by paper chromatography. 15. The branched-chain fatty acid synthesizing system uses branched-chain ?-keto acid decarboxylase ( BCKA ) enzyme. 16. Unlike related keto acids , it is not an intermediate or metabolite associated with amino acids and its origin is unknown. 17. The amino group is fed into the urea cycle, leaving a deaminated carbon skeleton in the form of a keto acid . 18. If the amino group is removed from an amino acid, it leaves behind a carbon skeleton called an ?-keto acid . 19. Several of these keto acids are intermediates in the citric acid cycle, for example the deamination of glutamate forms ?-ketoglutarate. 20. Semicarbazide stains ?-keto acids on the TLC plate, which must then be viewed under ultraviolet light to see the results.