11. During lagging strand synthesis, the replicative polymerase sends the lagging strand back toward the replication fork. 12. Replication on the leading and lagging strands is performed by DNA polymerase ? and DNA polymerase ?. 13. The interaction between DnaG and DnaB is necessary to control the longitude of Okazaki fragments on the lagging strand . 14. This asymmetry is due the formation of the replication fork and its division into nascent leading and lagging strands . 15. At this point, the lagging strand replicative polymerase associates with the clamp and primer in order to start polymerisation. 16. In DNA replication at the lagging strand site, DNA polymerase III removes nucleotides individually from the DNA binding protein. 17. Any deviation from parity rule 2 will result in asymmetric base composition that discriminates the leading from the lagging strand . 18. This polymerase synthesizes lagging strand DNA in eukaryotes . ( Thought to form an asymmetric dimer with DNA polymerase epsilon .) 19. When this is complete, a single nick on the leading strand and several nicks on the lagging strand can be found. 20. Priming occurs once at the origin on the leading strand and at the start of each Okazaki fragment on the lagging strand .
