In order to expose the nickel-binding hook, a leader sequence must be enzymatically cleaved off the N-terminus.
12.
The result is that the further downstream genes are from the leader sequence, the less they will be transcribed by RNA polymerase.
13.
That is, the further the gene is from the leader sequence, the greater the chance of RNA dimerization inhibiting RNA polymerase.
14.
If it dissociates, it must reenter the genome at the leader sequence, rather than continuing to transcribe the length of the genome.
15.
When paramyxovirus genome was exposed to UV light, the level of inhibition of transcription was proportional to the distance from the leader sequence.
16.
The 5-leader sequence has an internal ribosome entry site ( IRES ) and cap-independent translation regulatory elements ( CIREs ).
17.
After processing, the cleaved leader sequence of the iron-sulfur protein is retained as subunit 9, giving 11 subunits from 10 genes.
18.
These have names like "'CEOP5460 "'and are manually curated using evidence from the SL2 trans-spliced leader sequence sites.
19.
The 35S RNA of CaMV contains a ~ 600nt leader sequence which contains 7-9 short open reading frames ( sORFs ) depending on the strain.
20.
This long leader sequence has the potential to form an extensive complex stem-loop structure, which is an inhibitory element for expression of following ORFs.
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