11. These stages are prophase, prometaphase, metaphase , anaphase, and telophase. 12. Metaphase is now published under the Teamcenter Enterprise name by Siemens PLM Software.13. RNAi mediated depletion of human Bub1 has indicated function in correct metaphase congression. 14. It cells in metaphase and allowing cell harvest and karyotyping to be performed. 15. During cell division, demecolcine inhibits mitosis at metaphase by inhibiting spindle formation. 16. Chromosomes then orientate on the metaphase spindle for mitosis. 17. The cell goes through a prolonged delay in a metaphase -like state. 18. The end result is the metaphase chromosome. 19. At metaphase , a proteinaceous remnant of the nucleolus often remains associated with the secondary constriction. 20. Similarly, in C . elegans, condensin activity is dependent on Aurora B in metaphase .