This precludes the bidentate coordination complex necessary for di-phenolate hydroxylation characteristic of tyrosinase but absent in catechol oxidase.
12.
It has been postulated that a proton lost by the phenol group of 2, 4, 6-TBP resulting in the formation of a negatively charged halo-phenolate ion.
13.
Phenol is easily neutralized by sodium hydroxide forming sodium phenate or phenolate, but being weaker than carbonic acid, it cannot be neutralized by sodium bicarbonate or sodium carbonate to liberate carbon dioxide.
14.
Regular nucleophiles such as the fluoride anion, aniline, pyridine, ethylene diamine and the phenolate ion were found to have pseudo first order reaction rates corresponding to their basicity as measured by their pK a.
15.
The beta barrel structure is a nearly perfect cylinder, 42?long and 24?in diameter, Inward-facing sidechains of the barrel induce specific cyclization reactions in Ser65 Tyr66 Gly67 that induce ionization of HBI to the phenolate form and chromophore formation.
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