The column is first washed with a low concentration of sodium phosphate buffer, which elutes the single-stranded DNA, and then with high concentrations of phosphate, which elutes the double stranded DNA . The amount of DNA in these two solutions is then measured using a spectrophotometer.
12.
To prepare drug products for testing, they must be dissolved in certain substances based on their " physical characteristics . " For example, a water-soluble drug product should be dissolved in " Buffered Sodium Chloride-Peptone Solution pH 7.0, Phosphate Buffer Solution pH 7.2, or Soybean-Casein Digest Broth ."
13.
This question is something I'm thinking about as we add 5-10 mM calcium chloride to our amino-sulfonate ( HEPES etc . ) buffered immunoassay reagent in an attempt to combat non-selective binding ( false positives, etc . ) How sensitive our reagent would be to phosphate impurities leftover from the phosphate buffer used to make our solid phase magnetic particle reagent . . . we already carried out a buffer exchange . ) talk ) 18 : 10, 12 April 2016 ( UTC)
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