EcoRV is the only type II restriction endonuclease known to cause a major protein-induced conformational change in the DNA.
12.
The cloning vector is treated with a restriction endonuclease to cleave the DNA at the site where foreign DNA will be inserted.
13.
A D86G mutation of " EMG1 " destroys an EcoRV restriction endonuclease site in the most highly conserved region of the protein.
14.
In the case of large DNA molecules, the DNA is frequently cut into smaller fragments using a DNA restriction endonuclease ( or restriction enzyme ).
15.
A restriction endonuclease typically requires a recognition site and a cleavage pattern ( typically of nucleotide bases : A, C, G, T ).
16.
The crystal structure of Prp8 protein ( residues 885 2413 ) reveals tightly associated domains that resemble an intron reverse transcriptase and a type II restriction endonuclease.
17.
DNA samples before or after restriction enzyme ( restriction endonuclease ) digestion are separated by gel electrophoresis and then transferred to a membrane by blotting via capillary action.
18.
The positions of these two cuts, both in relation to each other, and to the recognition sequence itself, are determined by the identity of the restriction endonuclease.
19.
19 . Haertl R, Bandlow G . Epidemiological fingerprinting of Enterobacter cloacae by small-fragment restriction endonuclease analysis and pulsed-field gel electrophoresis of genomic restriction fragments.
20.
A Restriction endonuclease enzyme is extracted from Alu that acts at the centre of a palindromic tetranucleotide sequence to give even-ended duplex DNA fragments phosphorylated at the 5'- end.
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