11. Components of this relationship are fiber length, number of sarcomeres and pennation angle. 12. Accordingly, myopalladin has functions in both sarcomere assembly and in control of gene expression. 13. Calcium is also used in triggering the contraction of muscle fibres ( see Sarcomere ). 14. Within the sarcomere , actin and myosin fibers overlap in a contractile motion towards each other. 15. Sarcomeres are marked by Z lines which show the beginning and the end of a sarcomere.16. Sarcomeres are marked by Z lines which show the beginning and the end of a sarcomere . 17. The Ca2 + flows from the sarcoplasmic reticulum into the sarcomere with both of its filaments. 18. Upon stimulation by an action potential, skeletal muscles perform a coordinated contraction by shortening each sarcomere . 19. Calcium is released from the sarcoplasmic reticulum into the sarcomere when a muscle is stimulated to contract. 20. This protein makes up part of the sarcomere and forms macromolecular filaments composed of multiple myosin subunits.