By the time Prasher's ACS funding ended, he had isolated a partial, but almost complete " gfp " cDNA, with 965 bases out of the 1, 050 bases of the corresponding mRNA . It would require construction of another cDNA library during the following ( non-funded ) year for Prasher to isolate a full-length cDNA clone, although it must be noted that this partial cDNA clone was subsequently used and found to be sufficient for successful heterologous expression in " E . coli ", " C . elegans " and " A . thaliana ".
22.
By the time Prasher's ACS funding ended, he had isolated a partial, but almost complete " gfp " cDNA, with 965 bases out of the 1, 050 bases of the corresponding mRNA . It would require construction of another cDNA library during the following ( non-funded ) year for Prasher to isolate a full-length cDNA clone, although it must be noted that this partial cDNA clone was subsequently used and found to be sufficient for successful heterologous expression in " E . coli ", " C . elegans " and " A . thaliana ".
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