21. Linear excitation of fluorescence with confocal detection. 22. The system works with confocal microscopes, or image stacks that are suitable for stereology. 23. The protein expression data is derived from antibody-based profiling using immunofluorescence confocal microscopy. 24. LSFM combines good z-sectioning ( as confocal ) and only illuminates the observed plane 25. Tip-enhanced Raman spectroscopy requires a confocal microscope, and a scanning probe microscope. 26. Because STED selectively deactivates the fluorescence, it can achieve resolution better than traditional confocal microscopy. 27. Typically a confocal microscope is used to provide overlapping green and red focal volumes for excitation. 28. PI is suitable for fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry. 29. Compared to full sample illumination, confocal microscopy gives slightly higher resolution, and significantly improves optical sectioning. 30. The rise of fluorescence microscopy drove the development of a major modern microscope design, the confocal microscope.
