21. The equilibrium concentrations of reactants and products are related by the acid dissociation constant expression, ( K a ): 22. The acid dissociation constant for an acid is a direct consequence of the underlying Gibbs free energy change for the reaction. 23. There is a range of reported values for the dissociation constant of BAPTA, though 0.2 �M appears consistently. 24. Another way to provide acid dissociation constant data is to list pK a1, pK a2, and pK a3 instead. 25. In solution, 4-nitrophenol has a dissociation constant ( pK a ) of 7.15 at 25 �C. 26. NSTX and other site 1 blockers have high affinity ( very low dissociation constant ) and high specificity for Nav channels. 27. Where K _ d = k _ r / k _ f is the dissociation constant for the enzyme-substrate complex. 28. From the law of mass action, the apparent dissociation constant K _ d, an equilibrium constant, is given by: 29. Surface phase dissociation constant KDsurf ( 3.84?.68 ) is obtained by fitting Langmuir adsorption isotherm on equilibrium signals. 30. The smaller the dissociation constant , the more tightly bound the ligand is, or the higher the affinity between ligand and protein.
