21. In MEKC, however, techniques must be used to extend the elution range to separate strongly retained analytes. 22. Elution buffer is consistently being drawn from the chamber into the mixing chamber, thereby altering its buffer concentration.23. The Van Deemter equation was the result of the first application of rate theory to the chromatography elution process. 24. This results in a gentle separation of particles based on mass with the elution order of smallest to largest. 25. The Combo Dual Therapy Stent is a coronary stent that combines Genous with an antiproliferative, biodegradable sirolimus drug elution . 26. A change to elution buffer while maintaining upward flow results in desorption of the target protein in expanded-bed mode. 27. The mode used for elution ( expanded-bed versus settled-bed ) depends on the characteristics of the feed. 28. Elution ranges can be extended by several techniques including the use of organic modifiers, cyclodextrins, and mixed micelle systems.29. Additional columns can give additional flexibility for elution and regeneration times, at the cost of additional equipment and resin costs. 30. The final step involves the elution step where the bound phages are eluted through changing of pH or other environment conditions.
