21. The undiluted stain both acts as a fixative and also partially stains the smear. 22. The fixative is injected into the heart with the injection volume matching cardiac output. 23. Artwork media requiring fixative include drawings done in pencil, charcoal, and pastel. 24. Fixative is most commonly available in aerosol sprays.25. This fixative is an aqueous solution of formalin, potassium nitrate and potassium acetate. 26. This method involves adding a high concentration of formamide to a osmium tetroxide fixative . 27. Coumarin is a chemical fixative used in the preparation of detergents, cosmetics and perfumes. 28. Acrolein is sometimes used as a fixative in preparation of biological specimens for electron microscopy. 29. When the design is finished, a fixative is applied to protect the finished work. 30. Such a technique requires a workable fixative .
