This technique made it possible to study biologically active substances called monoamines dopamine, noradrenaline, adrenaline and serotonin on the cellular level employing fluorescence microscope.
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In order to monitor the fate of cytosolic misfolded proteins " in vivo ", a plasmid carrying a transformed into yeast and visualized using a fluorescence microscope.
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In microscopy, the development and refinement of the confocal microscope, the fluorescence microscope, and the total internal reflection fluorescence microscope all belong to the field of biophotonics.
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A "'fluorescence microscope "'is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, absorption to study properties of organic or inorganic substances.
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The fluorescent signal could be easily visualized using a fluorescence microscope and this allowed the rapid examination of the localization of molecules in cells and in tissues.
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In microscopy, the development and refinement of the confocal microscope, the fluorescence microscope, and the total internal reflection fluorescence microscope all belong to the field of biophotonics.
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Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources.
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A "'total internal reflection fluorescence microscope "'( "'TIRFM "') is a type of microscope with which a thin region of a specimen, usually less than 200 nm can be observed.
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Most fluorescence microscopes are operated in the Epi-illumination mode ( illumination and detection from one side of the sample ) to further decrease the amount of excitation light entering the detector.
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These microscopes are widely used in biology and are the basis for more advanced microscope designs, such as the confocal microscope and the total internal reflection fluorescence microscope ( TIRF ).
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