21. Because of its high quantum yield, B-PE is considered the world s brightest fluorophore . 22. If cleavage occurs, the fluorophore will be separated from the quencher molecule generating a detectable signal. 23. This enzyme has been found effective at improving organic fluorophore -stability in single-molecule experiments. 24. The choice of a particular fluorophore ultimately depends on the application and on its underlying photophysical properties. 25. Its fluorophore , and therefore its optical properties, are essentially identical to those of ethidium bromide. 26. Mathematical methods are used to restore spatio-temporal distribution of the thrombin from the fluorophore signal. 27. Another method of secondary antibody detection utilizes a near-infrared ( NIR ) fluorophore -linked antibody. 28. FRET will respond to internal conformational changes result from reorientation of the fluorophore with respect to the other. 29. For steady-state fluorescence measurements it is usually measured by embedding the fluorophore in a frozen polyol. 30. The antibody can either be conjugated to a fluorophore or to horseradish peroxidase for imaging and / or quantification.
