In nonhuman animals, " Babesia canis rossi ", " Babesia bigemina ", and " Babesia bovis " cause particularly severe forms of the disease, including a severe haemolytic anaemia, with positive erythrocyte-in-saline-agglutination test indicating an immune-mediated component to the haemolysis.
22.
Sequence studies have shown the protein to be similar both to alpha toxin from " Clostridium perfringens " and " Clostridium bifermentans ", a phospholipase C involved in haemolysis and cell rupture, and to lecithinase from " Listeria monocytogenes ", which aids cell-to-cell spread by breaking down the 2-membrane vacuoles that surround the bacterium during transfer.
23.
Acetoin, clumping factor and coagulase are not produced and activities of urease, haemolysis, arginine dihydrolase, arginine arylamidase, ornithine decarboxylase, beta-galactosidase, beta-glucuronidase, pyrrolidonyl arylamidase, esterase ( C4 ), lipase ( C14 ), Naphthol-AS-BI-phosphohydrolase, valine arylamidase, cystine arylamidase, alpha-galactosidase, N-acetyl-beta-glucosaminidase, alpha-mannosidase, alpha-fucosidase and esterase-lipase ( C8 ) are negative.
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