The capsule gene cluster of Lg2 may be a genomic island from several features such as the presence of insertion sequences flanked on both ends, different GC content from the chromosomal average, integration into the locus syntenic to other lactococcal genome sequences, and distribution in human gut microbiomes.
22.
This approach may not give the correct amino acid composition of the protein, in particular if unconventional amino acids such as selenocysteine are incorporated into the protein, which is coded for by a conventional stop codon in combination with a downstream hairpin ( SElenoCysteine Insertion Sequence, or SECIS ).
23.
"' RNA-OUT "'is a non-coding RNA that is antisense to the RNA-IN non-coding RNA . Transposition of insertion sequence IS10 is regulated by an anti-sense RNA which inhibits transposase expression when IS10 is present in multiple copies per cell.
24.
In order for UGA to be read as a selenocysteine amino acid instead of a stop codon, it is necessary that a downstream stem loop sequence, the selenocysteine insertion sequence ( SECIS ), be present to bind with SECIS binding protein-2 ( SBP-2 ), which binds with elongation factor EFsec . even though it is important to the functioning of the enzyme.
25.
PCR methods that have been developed are based on the 16S rRNA gene, the hsp65 gene, or the insertion sequence IS2404 . evaluated two nested PCRs : the nested IS2404-based PCR and the nested 16S rRNA gene-based PCR . IS2404-based PCR was positive only with " M . ulcerans " isolates and the closely related " M . shinshuense ".
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