21. The myrosinase enzyme turns the glucosinolates into various isothiocyanate compounds known generally as mustard oil. 22. That healthy-looking salad might offer up some allyl isothiocyanate , caffeic acid and estragole. 23. When an animal chews the plant, the allyl isothiocyanate is released, repelling the animal. 24. Sinalbin is metabolised to form the mustard oil 4-hydroxybenzyl isothiocyanate by the enzyme myrosinase. 25. Hot mustard and horseradish contain isothiocyanates , which vaporize and hit the trigeminal nerve in your nose. 26. An artificial isothiocyanate , phenyl isothiocyanate, is used for amino acid sequencing in the Edman degradation. 27. An artificial isothiocyanate, phenyl isothiocyanate , is used for amino acid sequencing in the Edman degradation. 28. Hence, the oxygen in the molecule stems from the hydroxy group of the original unstable isothiocyanate . 29. The heat comes from mustard oils, or isothiocyanates , not capsaicin, the fiery substance in chilies. 30. Its stems contain chemical compounds known as isothiocyanates , which give wasabi its famous sinus-clearing properties.