21. Ligase ribozymes may have been part of such a pre-biotic RNA world.22. Activation and recruitment of DNA Ligase I seem to be associated with posttranslational modifications. 23. This interaction is crucial for the recruitment of substrates to the ubiquitin ligase complex. 24. Most obviously, for example, formate-tetrahydrofolate ligase synthesizes 10-formyltetrahydrofolate. 25. DTX1 function as ubiquitin ligase protein by facilitating ubiquitination and allowing degradation of MEKK1. 26. Ligase works to fill these nicks in, thus completing the newly replicated DNA molecule.27. DNA ligase then joins the deoxyribonucleotides together, completing the synthesis of the lagging strand. 28. Once paired, the phosphodiester bonds of the fragments can be joined by DNA ligase . 29. Additionally, E3 SUMO-protein ligase PIAS is an alternative name for PIAS proteins. 30. However, it is not needed for E3 SUMO-protein ligase activity to occur.
