21. If the result is non-ligated , a virama is visible, attached to C 1. 22. These findings were observed in ligated carotid arteries of mice to mimic the effects of d-flow. 23. Cells containing successfully ligated insert can then be easily identified by its white coloration from the unsuccessful blue ones. 24. If the result is not ligated , a virama is visible, attached to C 1, actually written. 25. In each cycle, 8-mer probes are added and ligated according to their first and second bases. 26. Atjeh and others like him have been vasectomized, tubally ligated or implanted with heavy-duty birth control treatments. 27. With abdominoplasty, we're dealing more with the superficial veins which come up, get ligated and cut. 28. Ubiquitin contains seven lysine residues to which another ubiquitin can be ligated , resulting in different types of polyubiquitin chains. 29. "Self ligation " is a bracket that has a sliding or rotating mechanism to ligate an archwire. 30. In either case, the fragments are ligated into a vector that has been digested with the same restriction enzyme.