Pregnancy testing was conducted in the mid-20th century by injecting urine from a woman into a male toad's lymph sacs, and if spermatozoa appeared in the toad's urine, the patient was deemed to be pregnant.
22.
Previously, it was debated whether the "'lymph sacs "'developed from the venous system, or if they came from spaces in the mesenchyme, which come together in a centripetal direction and secondarily opened into the veins.
23.
There are many transcription factors that regulate the development of the lymphatic system, particularly the "'lymph sacs "', but in all of the migrating lymphatic endothelial cell precursors, there is one specific factor present, " Prospero-related homeobox-1 ( PROX1 ) ".
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