Highly virulent or lytic strains give clear plaques while strains that only kill a fraction of their hosts ( due to partial resistance / lysogeny ) or only reduce the rate of cell growth give turbid plaques.
22.
However, when the Hfr parent was lysogenic, lysogeny ( i . e ., the prophage ) was not inherited by any of recombinants, which were recovered by growing them as colonies on the appropriate agar medium.
23.
It is encoded in the lambda phage genome by the 291 base pair cII gene . cII plays a key role in determining whether the bacteriophage will incorporate its genome into its host and lie dormant ( lysogeny ), or replicate and kill the host ( lysis ).
24.
If cII levels reach a certain threshold level, expression through the promoters pI, pRE and pAQ will be induced, initiating the two actions necessary in establishing lysogeny : 1 ) repression of lytic genes and 2 ) integration of the phage genome into the host s chromosome.
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