21. Such a test is known as an MBAS assay ( methylene blue active substances assay ). 22. Methylene blue is reduced to colorless leucomethylene blue.23. Vital methylene blue was formerly much used for examining the innervation of muscle, skin and internal organs. 24. However dead cells are unable to reduce the oxidized methylene blue and the cells are stained blue. 25. Later, Robert Koch also used methylene blue as a dye in his research on the tuberculosis pathogen. 26. Malachowski used alkali-treated methylene blue solutions and Romanowsky used methylene blue solutions which were molded or aged. 27. Malachowski used alkali-treated methylene blue solutions and Romanowsky used methylene blue solutions which were molded or aged. 28. With Paul Ehrlich ( 1854-1915 ), he discovered that the histological stain, methylene blue had effectiveness against malaria. 29. That one is NaOH, glucose & methylene blue , which starts out deep blue, and gradually fades to colourless. 30. Methylene blue is a cationic dye.
