21. Yet, the Pitx 2 pathway is PAX3 dependent and requires this gene to enact limb myogenesis . 22. It accomplishes this by repressing MyoD transcription by binding to the enhancer region, and prevents premature myogenesis . 23. Pax7 is a transcription factor that plays a role in myogenesis through regulation of muscle precursor cells proliferation. 24. A lack of c-Met disrupts secondary myogenesis and as in LBX1 prevents the formation of limb musculature. 25. These consequences further reveal the complexity of myogenesis and the importance of each genetic factor in proper muscle development. 26. Pitx2 can determine the development and activation of the MyoD gene ( the gene responsible for skeletal myogenesis ). 27. Calponin 3 is also present in embryonic trophoblasts and myoblasts with functions in cell fusion during embryonic development and myogenesis 28. Skeletal myogenesis depends on the strict regulation of various gene subsets in order to differentiate the myogenic progenitors into myofibers. 29. This has also revealed the significance of PAX3 in myogenesis , and that it ensures the survival of myogenic progenitors. 30. Calponin 3 has been shown to participate in actin cytoskeleton-based activities such as that in embryonic development and myogenesis .
