21. In bacteria it is found in association with HNH, a non-specific nuclease motif. 22. Then, the cells are lysed and the DNA is sheared by sonication or using micrococcal nuclease . 23. In " M . smegmatis " the SSA pathway depends on the RecBCD helicase-nuclease . 24. N protein encapsidates genome RNA into a nuclease -resistant form to protect in from cellular RNAse function. 25. To address these problems, Rhee and Pugh revised the classic nuclease protection assay to develop ChIP-exo. 26. Moreover, the use of restriction enzymes such as the zinc-finger nuclease ( ZFN ) is being studied. 27. The ERCC1-XPF nuclease is an essential activity in the pathway of DNA nucleotide excision repair ( NER ). 28. He identified RuvC as the first cellular enzyme that resolves recombination intermediates and characterised how this nuclease cuts Holliday junctions. 29. Translation is halted via the addition of cycloheximide, and the DNA / RNA in the cell is then nuclease treated. 30. Primer extension offers an alternative to a nuclease protection assay ( S1 nuclease mapping ) for quantifying and mapping RNA transcripts.
