Self-activating prey, where the randomized region facilitate reporter expression in the absence of TF binding, are removed by transforming the reporter vector library into bacteria in the absence of bait and assaying for growth on plates containing 5-fluoro-orotic acid ( 5-FOA ).
22.
Diagnostic testing for OTC deficiency, or any individual with hyperammonemia involves plasma and urine amino acid analysis, urine organic acid analysis ( to identify the presence or absence of orotic acid, as well as rule out an organic acidemia ) and plasma acylcarnitines ( will be normal in OTC deficiency, but can identify some other causes of hyperammonemia ).
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