Nucleic acids have a smaller percentage of hydrogen atoms, which are the atoms usually observed in NMR, and because 31 P NMR . Two-dimensional NMR methods are almost always used, such as correlation spectroscopy ( COSY ) and total coherence transfer spectroscopy ( TOCSY ) to detect through-bond nuclear couplings, and nuclear Overhauser effect spectroscopy ( NOESY ) to detect couplings between nuclei that are close to each other in space.
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Nucleic acids have a smaller percentage of hydrogen atoms, which are the atoms usually observed in NMR spectroscopy, and because 31 P NMR . Two-dimensional NMR methods are almost always used, such as correlation spectroscopy ( COSY ) and total coherence transfer spectroscopy ( TOCSY ) to detect through-bond nuclear couplings, and nuclear Overhauser effect spectroscopy ( NOESY ) to detect couplings between nuclei that are close to each other in space.
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"' Correlation spectroscopy "'is one of several types of two-dimensional nuclear magnetic resonance ( NMR ) spectroscopy or 2D-NMR . This type of NMR experiment is best known by its acronym, COSY . Other types of two-dimensional NMR include J-spectroscopy, exchange spectroscopy ( EXSY ), Nuclear Overhauser effect spectroscopy ( NOESY ), total correlation spectroscopy ( TOCSY ) and heteronuclear correlation experiments, such as HSQC, HMQC, and HMBC . In correlation spectroscopy, emission is centered on the peak of an individual nucleus; if its magnetic field is correlated with another nucleus by through-bond ( COSY, HSQC, etc . ) or through-space ( NOE ) coupling, a response can also be detected on the frequency of the correlated nucleus.
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