In 1906 botanist Mikhail Tsvet invented paper chromatography, an early predecessor to thin layer chromatography, and used it to separate and examine the plant proteins that make up chlorophyll.
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The discovery in 1943 by Martin and Synge of paper chromatography provided for the first time the means of surveying the phenolic constituents of plants and for their separation and identification.
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A short abstract in 1943 followed by a detailed article in 1944 described the use of filter paper as the stationary phase for performing chromatography on amino acids : paper chromatography.
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In a 1903 lecture ( published in 1905 ), Tsvet also described using filter paper to approximate the properties of living plant fibers in his experiments on plant pigments & mdash; a precursor to paper chromatography.
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They established the principles and basic techniques of partition chromatography, and their work encouraged the rapid development of several chromatographic methods : paper chromatography, gas chromatography, and what would become known as high performance liquid chromatography.
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Others, such as Linus Pauling and his students, applied newly developed combinations of electrophoresis and paper chromatography to proteins subject to partial digestion by digestive enzymes to create unique two-dimensional patterns, allowing fine-grained comparisons of homologous proteins.
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This new enterprise grew quickly and became one of the leading manufacturers of apparatus and instruments in the fields of paper chromatography, liquid chromatography and gas chromatography, as well as some other instruments used in the biological sciences.
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While Mayr quickly soured on paper chromatography, Sibley successfully applied electrophoresis to egg-white proteins to sort out problems in bird taxonomy, soon supplemented that with DNA hybridization techniques the beginning of a long career built on molecular systematics.
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A significant limitation to paper chromatography is that it will not show exactly how much malic is still remaining in the wine, with the size of the " splotch " on the paper having no correlation to a quantitative figure.
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:: The question is hidden in the section title ( annoying but common ) and would appear to ask about the separation via paper chromatography of the various pigments in grape juice .-- talk ) 00 : 33, 7 January 2011 ( UTC)
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