21. TPA and plasmin are the key enzymes of the fibrinolytic pathway in which tPA mediated plasmin generation occurs. 22. TPA and plasmin are the key enzymes of the fibrinolytic pathway in which tPA mediated plasmin generation occurs. 23. Moreover, plasmin induces the generation of bradykinin in mice and humans trough high molecular weight kininogen cleavage. 24. PLAT is secreted by vascular endothelial cells and acts as a serine protease that converts plasminogen to plasmin . 25. In this scenario, PAI-1 inhibits uPA via active site binding, preventing the formation of plasmin . 26. Inflammatory stimuli that activate macrophages enhance the release of active TGF-? by promoting the activation of plasmin . 27. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. 28. The circulating enzyme plasmin , the main enzyme of fibrinolysis, cleaves the fibrin gel in a number of places. 29. The conversion of plasminogen to plasmin involves the cleavage of the peptide bond between Arg-561 and Val-562. 30. Plasmin is inactivated by proteins such as sterically shielded, thus substantially decreasing the plasmin's access to protein substrates.
