Blunt-ended PCR product normally lacks a 5'- phosphate, therefore it needs to be phosphorylated by treatment with T4 polynucleotide kinase.
22.
"They told me they wanted to construct a helix, a polynucleotide to rival Pauling's alpha helix, " he said.
23.
April 3, 2001 Laus; Reiner, Shapero; Michael H ., Tsavaler; Larisa Prostate tumor polynucleotide compositions and methods of detection thereof.
24.
In this latter system, the polynucleotide phosphorlyase gene regulates its own expression levels by a feedback mechanism that involves its activity upon the RNA structure.
25.
DNA's secondary structure is predominantly determined by base-pairing of the two polynucleotide strands wrapped around each other to form a double helix.
26.
However, a single-stranded polymer may form a double-stranded one by complementary polymerization, using a single-stranded polynucleotide as a template.
27.
"' Exonucleases "'are enzymes that work by cleaving nucleotides one at a time from the end ( exo ) of a polynucleotide chain.
28.
They proposed the so-called package model in which one type of a polymerase is sufficient and copies all polynucleotide chains that contain a special recognition motif.
29.
The binding of the polynucleotide adenylyltransferase responsible for actually synthesizing the tail is a necessary prerequisite for cleavage, thus ensuring that cleavage and polyadenylation are tightly coupled processes.
30.
It proceeds essentially like PCR . Due to the presence of the terminating nucleotide, every conceivable length of polynucleotide based on size will be produced in the reaction.
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