21. Unlike the other types, type IV restriction enzymes recognize and cut only modified DNA. 22. Restriction enzymes digest portions of the DNA, leaving short fragments.23. All three plasmids are digested with appropriate restriction enzyme and then allowed to ligate. 24. The second method employs the use of restriction enzymes and a recognized DNA sequence. 25. This would help digest sequences that are not being recognized by methylation-sensitive restriction enzymes . 26. Restriction enzymes are endonucleases from eubacteria and archaea that recognize a specific DNA sequence.27. There are numerous types of restriction enzymes , each of which will cut DNA differently. 28. DNA is digested with the restriction enzyme AluI, many 1109 base-pair fragments were obtained. 29. The idea behind using the same restriction enzymes is to create ligation later on. 30. Other viruses, such as bacteriophages T3 and T7, encode proteins that inhibit the restriction enzymes .
